Skip to main content
. 2016 May 1;39(5):1083–1095. doi: 10.5665/sleep.5760

Figure 2.

Figure 2

Thermogenetic screen for mutants with reduced sleep rebound. (A) Screen protocol: Insertion lines were crossed into the c584-Gal4, UASTrpA1 background and the flies were entrained at 21°C. Sleep deprivation was induced for 9 h (ZT12-ZT21) at 29°C, after which the flies were allowed to recover at 21°C. The PySolo sleep profile presented is average sleep of all flies from a representative group of lines run in the screen. Y-axis represents fraction of time asleep in a 30-min bin. (B) Overall screen schematic: Flow chart describing the number of insertion lines selected at each screening stage. (C) Histogram showing rebound sleep (sleep amount on the recovery day subtracted from sleep amount on the baseline day Zeitgeber time (ZT)21-ZT12) for all the lines tested in the screen. Candidates for rescreening (< 2.5 percentile) are boxed. (D) Sleep rebound hits: After rescreening, two lines, MI00323/+ and MI00393/+, show reduced rebound after sleep deprivation. Plotted are sleep loss (SL) and sleep recovered (SR) from four independent experiments for MI00323/+ and MI00393/+ compared to all MiMIC insertions tested as heterozygotes in the screen. Error bars represent standard deviation. (E) Sleep profile for MI00323/+ and MI00393/+ during the thermogenetic sleep deprivation protocol in a representative experiment.