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. 2016 Apr 19;7:511. doi: 10.3389/fpls.2016.00511

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Copyright © 2016 Liu, Xu, Lou, Fan, Yang and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histochemical analysis of GUS expression in transgenic Arabidopsis lines driven by different 5′ deletion sequence of VuMATE1 promoter. (A) Induction of VuMATE1 by Al in transgenic Arabidopsis carrying VuMATE1p::GUS constructs. The representative images show GUS staining in roots after 9 h of exposure to 0 (-Al) or 1 μM Al (+Al) activity in nutrient solution. (B) GUS expression in root apex and maturation root zone of transgenic Arabidopsis carrying the shortest promoter of VuMATE1 (-192 bp::GUS). At least two independent transgenic lines were used to analyze GUS expression. Bar, 100 μm.