Table 2.
Primers used for PCR amplification and detection.
| Primers | Primers sequence (5′−3′)a | Amplification for |
|---|---|---|
| Gup-F | CTTCAAGCTTGCTTTTGTGGACTTA | Upstream border of gidA |
| Gup-R | GTTTGTCGACTCATGTTGTTCTCTCCT | |
| Gdown-F | GATCCCGGGGGCTGTTCTTTTCGC | Downstream border of gidA |
| Gdown-R | CCCCGAATTCTTCCTTGACCACAACC | |
| Erm-F | GTCTGGATCCCTTAGAAGCAAACTTAA | Erm r gene |
| Erm-R | GTTAGGATCCATCGATACAAATTCCCCG | |
| GidA-F | CGGGATCCATGACACACACATTTGCAGA | gidA gene |
| GidA-R | CGCTCGAGTTAGTGACTGTCCTTTGATTT | |
| 2162-F | GTGATGAAAAGATTTCGATT | Downstream gene of gidA |
| 2162-R | TTATCCAAAGTCAAGCCA | |
| 2164-F | GGTTGATTATAAAAGATGG | Upstream gene of gidA |
| 2164-R | TCATGTTGTTCTCTCCTT |
a
Underlined nucleotides denote enzyme restriction sites.