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. 2016 Apr 19;7:450. doi: 10.3389/fpls.2016.00450

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Copyright © 2016 Bajaj, Srivastava, Nath, Tripathi, Bharadwaj, Upadhyaya, Tyagi and Parida.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic depicting the major steps followed in an agarose gel-based EcoTILLING assay for efficient identification of functionally relevant molecular tags governing useful agronomic traits in chickpea. This strategy is optimized for successful large-scale mining of novel SNP allelic variants from the target genomic regions (genes) by genotyping in a constituted field-phenotyped association panel (desi and kabuli core/mini-core germplasm lines). A, Accessions; SP, Superpool; F, (Forward); and R, (Reverse) primers.