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. 2016 Mar 22;135(5):499–511. doi: 10.1007/s00439-016-1656-8

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — AmpliSeq exome properties and target region analysis. a Length density distribution calculated from the dimension of amplicons generated with the AmpliSeq Exome kit as determined from the provided BED. In the kit design, most of the CDS exons are covered by a single amplicon (b). The comparison of target regions across 6 different enrichment kits (c) revealed that no one fully address all the human CDS nor relevant clinical genes. The bar graph represents the number of genes with at least one exon partially addressed (green) or completely missed (red). Compared exome capture kits include: AmpliSeq Exome and TargetSeq Exome (Life Technologies), SureSelect Human All Exon v5/v6 (Agilent Technologies) and SeqCap EZ v2/v3 (Roche)