Table 1.
HPLC conditions
| Compound | Eluent | Flow rate (mL/min) | Retention time (min) |
|---|---|---|---|
| Preparative | |||
| CB184 | Acetonitrile:H2O (6:4) | 6 | 9.1 |
| CB190 | Acetonitrile:H2O (55:45) | 6 | 10.1 |
| Analytical | |||
| CB185 | Acetonitrile:H2O (7:3) | 1 | 7.8 |
| CB190 | Acetonitrile:H2O (65:35) | 1 | 8.0 |
Conditions employed for preparative and analytical HPLC are shown. Preparative HPLC was carried out using a Capcellpak C18 UG120S-5 μm column (10 mm inner diameter (i.d.) × 250 mm, Shiseido, Tokyo, Japan) and a JASCO HPLC system (JASCO, Tokyo, Japan) installed within the radiosynthesis system. Analytical HPLC was conducted using a Capcellpak C18 UG120S-5 μm column (4.8 mm i.d. × 250 mm, Shiseido, Tokyo, Japan) and a Shimadzu HPLC system (Shimadzu, Kyoto, Japan) with gamma ray detection using NaI(Tl) scintillator