Skip to main content
. 2016 Apr 15;7:11301. doi: 10.1038/ncomms11301

Figure 3. DpnI cleavage assay on fully- or hemi-methylated GATC and CATC/GATG DNA probes.

Figure 3

The PAGE gel shows the formation of digested products. (a) DNA probes containing fully methylated GATC (F-GATC, 10 pmol) or hemi-methylated GATC (H-GATC, 10 pmol) were treated with DpnI (10 units) for 30 min or overnight at 37 °C. (b) DNA probes containing fully methylated CATC/GATG (F-CATC, 10 pmol) or hemi-methylated CATC/GATG (H-CATC, 10 pmol) were treated with DpnI for 30 min or overnight. All sequences used are listed in Supplementary Table 1.