Figure 1.

Increased ROS Production in Aging Flies Correlates with Mitochondrial Dysfunction

(A) Representative images of dissected fly brains stained with MitoSOX from wild-type flies of the indicated ages.

(B) Quantification of (A) (n = 5).

(C) Representative EM images of Dahomey flight muscle sections at 1,000x magnification dissected at the indicated ages (n = 10, 1 muscle per fly; red arrows indicated exemplar swollen, rounded mitochondria, see Figure S1C for quantification).

(D) Mitochondrial respiration in Dahomey and Oregon R flies at the indicated ages (n = 6).

(E) CI and CIII enzymatic activity in wild-type flies of the indicated ages (n = 5).

(F) CI (NDUFS3), CII (SDHB), CIII (CYTOB), CIV (COX4), and CV (ATP5A) levels in wild-type flies. GAPDH is used as a loading control.

(G) Quantification of (F).

(H) ImpL3 expression in wild-type flies of the indicated ages.

Values shown represent means ± SEM of at least three biological replicates, unless otherwise stated. See also Figure S1.