Figure 3.
CH Profiles and Lipoprotein Analyses of the Plasma of the SYN and LERKO Females
Plasma was obtained from SYN and LERKO females at 3 months of age euthanized at different phases of the estrous cycle or 30 days after OVX.
(A) Representative profile of the total CH content (expressed as milligrams per deciliters) in the fractions of plasma separated by FPLC. The experiment was repeated three times with six different animals in each experimental group.
(B and C) Western blot for apo-AI (B) and apo-E (C) in the FPLC fractions of the plasma at P and E.
(D) Sizes of HDLs (d = 1.063–1.21 g/ml) purified by sequential ultracentrifugation from pooled plasma samples. The data indicate mean ± SEM; n = 3 pools of plasma (each pool was composed of the plasma of six mice).
(E) Real-time PCR quantitative analyses of the liver mRNA contents of Pltp (top) and Lipc (bottom). The data indicate mean ± SEM; n = 6. The experiment was repeated twice.
(F) CEC as measured by radioisotopic assay in J774 cells pre-radiolabeled with 3H-CH and incubated with plasma from either SYN or LERKO females at P or E. The data are expressed as the percentage of the radioactivity released into the medium over the total radioactivity incorporated by the cells. The data indicate mean ± SEM; n = 10.
∗p < 0.05 and ∗∗∗p < 0.001 versus SYN at P; #p < 0.05, ##p < 0.01, and ###p < 0.001 versus SYN.