Fig. 4.

CCND1 and MYC was down-regulated with MALAT1 knockdown. U87 and U251 cells were seeded in 60 mm plates and grow to a confluency of 70%, then both cells were transfected with si-MALAT1 or Scramble respectively. QRT-PCR and western blot analysis were employed to verify the change of expression levels of CCND1 and MYC in response to MALAT1 knockdown in U87 cells (A, B) and U251 cells (C, D). The cells transfected with Scramble was used as the negative control.

*P < 0.05 vs. the control; ^† P < 0.01 vs. the control.