Skip to main content
. 2016 Apr 5;2016:1980763. doi: 10.1155/2016/1980763

Table 1.

Primers used for quantitative real-time RT-PCR.

Gene Sequence 5′→3′ Temperature profile
PiC GTGGTTTGGCTAAAGGATGG
GGGCAATGTCAGCGAAGAA
Denaturation 95°C, 15 s
Annealing 60°C, 40 s
Extension 72°C, 30 s

β-actin CGTAAAGACCTCTATGCCAACA
AGCCACCAATCCACACAGAG
Denaturation 95°C, 15 s
Annealing 60°C, 40 s
Extension 72°C, 30 s