Table 1.
Gene | Sequence 5′→3′ | Temperature profile |
---|---|---|
PiC | GTGGTTTGGCTAAAGGATGG GGGCAATGTCAGCGAAGAA |
Denaturation 95°C, 15 s |
Annealing 60°C, 40 s | ||
Extension 72°C, 30 s | ||
| ||
β-actin | CGTAAAGACCTCTATGCCAACA AGCCACCAATCCACACAGAG |
Denaturation 95°C, 15 s |
Annealing 60°C, 40 s | ||
Extension 72°C, 30 s |