Figure 4.

Histological comparison of microcirculation in the separated cerebellar layers and the midbrain in the wild type (A–C) and Lurcher (D–F) mice. (A,D)—The complete absence of Purkinje cells (red arrow) with intensive degeneration of the molecular (ML) and granular (GL) layers in Lurcher mice; (B,E)—Morphological features of vascularity in the white matter (WM) and nuclei (N). The boundary between the molecular and granular layers marked by the purple dotted lines; between the granular layer and white matter by the green and between the white matter and the nuclei by the blue dotted lines. (C,D)—No significant difference in the midbrain vascularity. Immunohistochemical detection of the microvessel laminin outlines, visualization with horseradish peroxidase/diaminobenzidine (brown), counterstaining with haematoxylin. Scale bar: 50 μm, uniform magnification in (A–F).