Table 1.
Independent variables and levels used for CCF design.
| Variables | Level | ||
|---|---|---|---|
| −1 | 0 | 1 | |
| Solid buffer pka | MOPS (7.2) | TAPS (8.4) | CAPSO (9.6) |
| Enzyme loading (PLU.mmol-1) | 55 | 170 | 285 |
| LGO concentration (M) | 0.5 | 0.75 | 1 |
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