Table 2.
Central composite face-centered (CCF) design and experimental responses.
| Run/Replicate a | Solid buffer (pka) | Enzyme loading (PLU.mmol−1 of LGO) | [LGO] (M) | LGO conversion (%) | Enzyme residual activity (%) |
|---|---|---|---|---|---|
| x1 | x2 | x3 | Y1 | Y2 | |
| 1/18 | MOPS (7.2) | 55 | 0.5 | 57.4/68.0 | 88.5/82.0 |
| 2/19 | CAPSO (9.6) | 55 | 0.5 | 79.9/79.7 | 63.6b/37.6 |
| 3/20 | MOPS (7.2) | 285 | 0.5 | 81.3/87.0 | 85.3/81.0 |
| 4/21 | CAPSO (9.6) | 285 | 0.5 | 87.5/90.8 | 52.7/57.3 |
| 5/22 | MOPS (7.2) | 55 | 1.0 | 74.8/74.5 | 70.3/76.9 |
| 6/23 | CAPSO (9.6) | 55 | 1.0 | 47.3/63.9 | 60.2/60.7 |
| 7/24 | MOPS (7.2) | 285 | 1.0 | 81.5/91.6 | 51.2/44.8 |
| 8/25 | CAPSO (9.6) | 285 | 1.0 | 87.2/90.4 | 69.2/52.4b |
| 9/26 | MOPS (7.2) | 170 | 0.75 | 84.4/87.2 | 72.4/73.4 |
| 10/27 | CAPSO (9.6) | 170 | 0.75 | 90.0/88.9 | 68.9/67.0 |
| 11/28 | TAPS (8.4) | 55 | 0.75 | 73.9/82.0 | 73.1/63.1 |
| 12/29 | TAPS (8.4) | 285 | 0.75 | 67.8b/91.3 | 74.8/50.6 |
| 13/30 | TAPS (8.4) | 170 | 0.5 | 86.6/87.6 | 61.8/61.1 |
| 14/31 | TAPS (8.4) | 170 | 1.0 | 88.4/89.5 | 12.6b/67.5 |
| 15/32 | TAPS (8.4) | 170 | 0.75 | 87.9/90.2 | 65.7/68.5 |
| 16/33 | TAPS (8.4) | 170 | 0.75 | 93.8/90.3 | 74.9/74.1 |
| 17/34 | TAPS (8.4) | 170 | 0.75 | 92.4/89.9 | 76.8/75.8 |
| ADDITIONAL RUNS | |||||
| 35/36 | MOPS (7.2) | 152 | 0.70 | 78.0/80.8 | 78.2/79.4 |
| 37/38 | CAPSO (9.6) | 80 | 0.94 | 72.5/73.6 | 70.0/71.6 |
| 39 | HEPES (7.5) | 120 | 0.65 | 79.7 | 76.5 |
a
Runs performed in a totally random order.
b
Outlier: observation point that is distant from other observations. Excluded from the data set.