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PrOF NMR titration of (+)-JQ1 with Brd4(1). (A) Bottom to top: 5FW-Brd4(1) (25 μM), with 1 equiv (+)-JQ1, with 5FW-BPTF (25 μM), with 5FW-BPTF (25 μM) and 1 equiv (+)-JQ1, with unlabeled BrdT(1) (50 μM) and 1 equiv (+)-JQ1. (B) Bottom to Top: 3FY-Brd4(1) (47 μM) titrated with 2 equiv (+)-JQ1.
