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. 2016 Apr 19;6:24675. doi: 10.1038/srep24675

Figure 3. Combination of OXA and Curcumin in OXA-sensitive and –resistant CRC cell lines.

Figure 3

(a) Graphic representation of the Combination Index (CI) values corresponding to different OXA plus Curcumin (CURC) treatment schedules in the HTOXAR3 cell line. (b) Bar graphs representing mean ± SEM percentage of cell viability after a 24-hour treatment with OXA, Curcumin or their concomitant combination at the indicated doses in HT29 and HTOXAR3 cells (c). *p-value < 0.05; **p-value < 0.01; ***p-value < 0.001 relative to the indicated treatment condition. (d) OXA doses (mean ± SEM) corresponding to indicated inhibitory concentrations (IC) in HT29 and HTOXAR3 cells as a single agent or when combined with Curcumin concomitantly for 24 h. (e) OXA and Curcumin doses (mean ± SD) corresponding to the indicated inhibitory concentrations (IC) when given as single agents or in a 24 h-concomitant schedule in HT29 and HTOXAR3. CI represents the combination index values in each case. Synergistic values (CI < 1) are highlighted in green. (f) Bar graph representing the percentage (mean ± SEM) of colonies in HT29 and HTOXAR3 cells after 24 h of the indicated treatments. *p-value < 0.05; **p-value < 0.01; ***p-value < 0.001 relative to NT (Non-treated cells). #p-value < 0.05 relative to OXA individual treatment. *p-value < 0.05 as compared to HT29. (g) Bar graph representing the percentage (mean ± SEM) of dead cells after 24, 48 and 72 h of treatment with OXA, Curcumin or their combination (as compared to non-treated controls) at their corresponding IC50 doses in HT29 and HTOXAR3 cell lines. *p-value < 0.05; **p-value < 0.01, relative to NT. #p-value < 0,05, relative to Curcumin individual treatment.