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. 2016 Apr 19;6:24675. doi: 10.1038/srep24675

Figure 4. Effect of Curcumin on constitutive and OXA-induced NF-κB activation.

Figure 4

(a) Western blot images of phosphorylated p65 (p-p65 Ser536), total p65, Survivin and Bcl-2 in HT29 and HTOXAR3 cells after a 24-hour treatment with 10 and 20 μM Curcumin (CURC). (b) Representative western blot images showing protein expression changes as indicated, in HT29 and HTOXAR3 cells after 24 h treatment with OXA or concomitant OXA plus Curcumin, for 0 to 120 minutes or 24 hours (c,d) Western blots showing p65 protein expression in cytoplasmic and nuclear extracts of HT29 cells after treatment with OXA (10 μM) or OXA + Curcumin (10 μM each) for short-time exposure (0 to 30 min) or 24 h (e). TNFα was used as positive control at 50 ng/ml. Msh2 and α-tubulin were used as nuclear- and cytoplasmic-specific protein controls, respectively. Images are representative of at least 3 independent experiments. NT: Non-treated cells.