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. 2016 Apr 19;6:24675. doi: 10.1038/srep24675

Figure 6. CXCL1, CXCL8 and CXCL2 gene expression and CXCL1 and CXCL8 secreted protein levels after treatment with OXA, Curcumin or both.

Figure 6

(a) Bar graphs illustrating relative gene expression levels (mean ± SEM) of the indicated chemokines in non-treated (NT) and after OXA or OXA plus Curcumin (CURC) treatment at IC50 doses in HT29 and HTOXAR3 cells. Gene expression levels of β-actin were used as endogenous control. *p-value < 0.05 relative to NT, #p-value < 0.05; ##p-value < 0.01, relative to HT29. ** p-value < 0.05 relative to OXA. (b) Changes in chemokines’ gene expression after treatment with Curcumin at indicated doses. (c) Graph bars showing levels of secreted chemokines (mean ± SEM) measured by enzyme-linked immunosorbent assay (ELISA) in the supernatants of treated cells as indicated. *p-value < 0.05; **p-value < 0.01; ***p-value < 0.001 relative to NT. ##p-value < 0.01, ###p-value < 0,001 relative to OXA. Results were obtained from at least 3 independent experiments.