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. Author manuscript; available in PMC: 2017 Jan 4.
Published in final edited form as: Curr Protoc Mol Biol. 2016 Jan 4;113:14.24.1–14.24.15. doi: 10.1002/0471142727.mb1424s113

Figure 2.

Figure 2

(A) Strategy for tagging a gene of interest with MS2 or PP7 loops. As an example, tagging in the 3’ UTR is shown, but the same applies to tagging in the 5’ UTR or in the intron. See text for details. (B) Examples of PCR reactions on a template containing PP7/MS2 repeats using Taq polymerase (left) or a high-fidelity (HF) polymerase (right).