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Induction of transcription of GAL10, tagged with PP7 at the 5’ UTR. (A) Before induction there is no transcription site, and only background levels of coat protein are visible. (B) Upon induction with galactose, GAL10 transcription sites appear. (C) After long induction, the coat protein is depleted from the nuclear, resulting in loss of visibility of the transcription sites and nuclear staining. Shown are maximum intensity projections of 9 z-planes, 150 ms exposure.
