Table 1.

Effects of mutations at α4S336 in the functional properties of α4β2 nAChRs expressed in Xenopus oocytes

AChR type (α4β2)	ACh			Nicotine
	EC50 ± SEM (µM)	Hill coefficient	n	EC50 ± SEM (µM)	Hill coefficient	n
Wild-type	1.1 ± 0.1	0.97 ± 0.1	9	2.5 ± 0.1	1.6 ± 0.7	7
α4S336A	8.6 ± 0.2*	0.4 ± 0.1*	9	1.8 ± 0.1	1.7 ± 0.6	5
α4S336D	17.4 ± 1.4*	0.6 ± 0.4*	5	2.0 ± 0.1	1.4 ± 0.3	13
	137.7 ± 0.1*	2.2 ± 2.7*

Values are given as the mean ± SEM.

^*Indicates P < 0.05, n indicates the number of oocytes tested