Table 2.
Potential Gene Transcription Biomarkers of Daphnia Exposure to Firemaster 550a
| A. genes differentially expressed in all Firemaster 550 exposures | ||||||
|---|---|---|---|---|---|---|
| gene ID | 0.243 mg/L | 0.0486 mg/L | 0.0486 µg/L | 0.243 µg/L | 0.0486 ng/L | gene function |
| DM05947 | −1.3 | −1.3 | −1.3 | −1.3 | −2 | peroxidase |
| DM12670 | −1.1 | −1.6 | −2.3 | −2 | −2.3 | unknown |
| DM09101 | −1.1 | −1.6 | −1.9 | −1.8 | −2.2 | ABC transporter |
| B. genes associated linearly with FM550 concentration | ||||||
|---|---|---|---|---|---|---|
| gene ID | trend | slope | rawp | Holm | BH | gene function |
| DM02757 | negative | −0.0129 | 6.7 × 10−05 | 0.0226 | 0.0226 | eukaryotic translation initiation factor |
| DM12108 | negative | −0.0328 | 1.9 × 10−04 | 0.0646 | 0.0324 | unknown |
| DM12547 | negative | −0.0032 | 4.0 × 10−04 | 0.1343 | 0.0450 | unknown |
a
RNA sequences from the microarray (Gene ID) were functionally annotated with Blast2Go. Part A shows log2 change of differential gene transcription in exposed Daphnia magna compared to control. Part B lists genes that showed a small but significant negative linear trend with increasing concentration. Holm: correction for multiple comparisons. BH (Benjamini–Hochberg procedure): controls false discovery rate.