Table 4.

Molecular Pathway Analysis on Metabolomic Data from FM550-Exposed Daphids^a

pathway analysis of Firemaster 550 metabolomic data
		increased metabolites
	totalb	expectedc	hitsd	raw pe	Holm adjustf	FDRg	organismh
nitrogen metabolism	9	0.047	2	8.29 × 10−04	0.067	0.067	D.r.h
arginine and proline metabolism	43	0.225	2	0.019	1	0.699	D.r.
aminoacyl-tRNA biosynthesis	67	0.350	2	0.044	1	0.749	D.r.

		decreased metabolites
	totalb	expectedc	hitsd	raw pe	Holm adjustf	FDRg	organismh
valine, leucine and isoleucine biosynthesis	13	0.090	3	5.62 × 10−05	0.004	0.004	D.r. and D.m.
aminoacyl-tRNA biosynthesis	67	0.463	4	5.28 × 10−04	0.041	0.021	D.r. and D.m.
valine, leucine and isoleucine degradation	35	0.242	3	0.001	0.093	0.032	D.r. and D.m.

^aAnalysis was performed with MetaboAnalyst 2.0 and included D. rerio (D.r.) and D. melanogaster (D.m.) pathways with more than one hit and a raw p-value <0.05.

^bTotal genes in biological pathway.

^cNumber of genes expected in a random data set.

^dNumber of hits in FM550 data.

^ep-Value calculated from raw data.

^fp-Value adjusted for multiplicity.

^gFalse discovery rate.

^hOrganism metabolome that resulted in significant findings.

^hAlso seen in D. melanogaster. If pathway was affected in both species, numbers represent data for the more significant species.