Table 5.

Enrichment Analysis on Metabolomic Data from FM550-Exposed Daphnids Compared to All Known Metabolites in the MetaboAnalyst Database^a

enrichment analysis of Firemaster 550 metabolomic data
		increased metabolites
	totalb	expectedc	hitsd	raw pe	Holm pf	FDRg
ammonia recycling	18	0.153	2	0.009	0.709	0.394
protein biosynthesis	19	0.161	2	0.010	0.779	0.394

		decreased metabolites
	totalb	expectedc	hitsd	raw pe	Holm pf	FDRg
protein biosynthesis	19	0.161	4	6.77 × 10−06	0.001	0.001
valine, leucine and isoleucine degradation	36	0.305	3	0.002	0.187	0.095

^aBiological functions with more than one hit and with raw p ≤ 0.05 are included as significant.

^bTotal genes in biological pathway.

^cNumber of genes expected in a random data set.

^dNumber of hits in FM550 data.

^ep-Value calculated from raw data.

^fp-Value adjusted for multiplicity.

^gFalse discovery rate.