Figure 3. Levels of DP₁, DP₂ and L-PGDS in primary cultured rat hippocampal neurons detected by reverse transcription polymerase chain reaction.

(A) The expressions of L-PGDS, DPs mRNA were measured by RT-PCR. The relative mRNA level of L-PGDS, DPs were standardized to endogenous β-actin mRNA for each sample. Al administration caused the significant increase of L-PGDS, DP₁ levels and decrease of DP₂ level compared with the control group. (B) The expressions of L-PGDS, DPs proteins were measured by WB. The relative protein levels of L-PGDS, DPs were standardized to endogenous β-actin protein for each sample. Al administration caused the significant increase of L-PGDS, DP₁ levels and decrease of DP₂ level compared with the control group. Values were mean ± SD (n = 3, **P < 0.01 vs. control group, one-way ANOVA with Dunnett’s multiple comparisons).