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Myosin heavy chain (MyHC) type 2a fiber frequency is reduced after 14 days of bed rest. A: representative immunohistochemical image of MyHC type 1 (pink), type 2a (green), and type 2x (orange) fiber identification. Scale bar = 100 μm. B: quantification of MyHC fiber type from immunohistochemical analysis, presented as mean fiber type frequency ± SE. *Significantly different from pre-bed rest (Pre-BR) value (P < 0.05).
