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. 2015 Dec 9;310(8):F705–F716. doi: 10.1152/ajprenal.00503.2015

Fig. 7.

Fig. 7.

Delayed treatment with UPHD186 accelerates recovery and reduces postinjury fibrosis after IR-AKI. Male BALB/c mice underwent left-sided renal pedicle clamping for 31 min to induce IR-AKI and then were treated with either vehicle or 50 mg/kg UPHD25 or 186 ip daily for 4 days, starting 3 days after injury. Mice underwent a right nephrectomy on day 8, and injured kidneys were harvested 28 days after injury and evaluated for markers of renal fibrosis. A–D: UPHD186 (injured vehicle controls, n = 13; UPHD186 treatment, n = 12). E–H: UPHD25 (injured vehicle controls, n = 7; UPHD25 treatment, n = 8). A and E: serum creatinine values 9 days after injury, 1 day after right nephrectomy to assess functional recovery. Individual data points and means for each group are shown. B and F: expression of renal fibrosis markers Col1α1, α-SMA, and LoxL2 mRNA relative to Gapdh mRNA control. C and D, G and H: Sirius red staining for interstitial collagen. C and G: quantification of Sirius red staining (% total area). D and H: representative images for Sirius red-stained tissues (outer medulla; scale bars = 50 μm). Values are means ± SE by 2-tailed t-test vs. vehicle controls. *P < 0.05. **P < 0.01. ***P < 0.001.