Fig. 1.

Establishment of maximally effective drug doses used in this study. A: concentration of compound A excreted in the urine over 4-h period (n = 3) as measured by mass spectrometry. Total urine was collected and measured at 2-h (B) or 4-h (C) time points following oral administration of bumetanide (50 and 100 mg/kg, n = 3 each), hydrochlorothiazide (HCTZ; 100 and 200 mg/kg, n = 3 each), amiloride (10 and 20 mg/kg, n = 4 each), benzamil (10 and 20 mg/kg, n = 3 each), or the vehicle (n = 6) alone. n.s., Not significant. %P < 0.05, significantly different from vehicle alone. *P < 0.05, significantly different from all other drug treatments.