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Effects of 24 h of FBS treatment or FBS + CX-5461 on myotube total RNA content (A), protein synthesis measured via the surface sensing of translation (SUnSET) method (B), and myotube volume (G). C–F: representative puromycin immunoblots (C) and ×10 immunocytochemical images (myosin heavy chain stained green; D–F). *Significantly different from control (P < 0.05).
