Figure 24.
Detection of mitophagy in primary cortical neurons using mitochondria-targeted Keima. Neurons transfected with mito-Keima were visualized using 458-nm (green, mitochondria at neutral pH) and 561-nm (red, mitochondria in acidic pH) laser lines and 575-nm band pass filter. Compared with the control (A) wild-type PINK1 overexpression (B) increases the number of the mitochondria exposed to acidic conditions. Scale bar: 2 µm. (C) Quantification of red dots suggests increased mitophagy in wild-type PINK1 but not in the kinase dead (kd) PINK1K219M-overexpressing neurons. Image provided by V. Choubey and A. Kaasik.