Figure 3.

Effect of SIDT2 overexpression on RNautophagy in the absence of LAMP2. (A) LAMP2 levels in LAMP2-deficient HeLa cells and parental HeLa cells (control HeLa) were analyzed by immunoblotting. (B and C) RNA uptake assays were performed using isolated lysosomes derived from LAMP2-deficient HeLa cells (B) or parental HeLa cells (C). Relative levels of RNA uptake were quantified. Results are expressed as mean ± SEM (n = 3). **, P < 0.01. In the absence of LAMP2, SIDT2 increased RNautophagy at similar levels to in the presence of LAMP2.