Figure 1.

Model for the formation and release of autophagic vesicles. GYPA (extracellular domain [red circle] and intracellular domain [green square]) is internalized by endocytosis of the plasma membrane (i) to produce large GYPA-decorated vesicles (ii) that fuse with an LC3 (blue pentagon)-positive autophagosome (iii) forming a large LC3-GYPA-positive hybrid inside-out vesicle (iv). Temporary weakening of the cytoskeleton (v) allows extrusion (vi) releasing a PS-positive inside-out vesicle (vii) for phagocytosis in the spleen.