Figure 4.

Interaction of BECN1(1–150)[4CS]Y and the BECN1(BARA/ECD) domain. (A) Coomassie-stained SDS-PAGE gel showing peak fractions following gel-filtration chromatography of BECN1(BARA/ECD) domain demonstrating it is very pure. (B) Superimposed ¹H-¹⁵N HSQC spectra of BECN1(1–150)[4CS]Y in the absence (black) and presence of BECN1(BARA/ECD) (red). Molar ratio of BECN1(1–150)[4CS]Y:BECN1(BARA/ECD) domain is 1:1. The reference spectrum (black) was acquired by mixing the BECN1(1–150)[4CS]Y with the equivalent volume of the buffer solution used for preparation of the BARA/ECD domain. (C) Ratio of peak intensities of BECN1(1–150)[4CS]Y in the presence of BECN1(BARA/ECD) to those in its absence plotted against the sequence of BECN1(1–150)[4CS]Y.