Skip to main content
. 2016 Apr 6;12(4):689–702. doi: 10.1080/15548627.2016.1151580

Figure 5.

Figure 5.

Interaction between OPA1 and FUNDC1 is important for mitophagy. (A) HeLa cells were transfected with FUNDC1-MYC or mutants and GFP-LC3 for 24 h. The cells were then fixed and immunostained to detect MYC (red) and HSP60 (purple). Scale bar: 10 µm. (B) The GFP-LC3 aggregates in cells treated as in (A) were quantified with imageJ. The GFP-LC3 aggregation area versus whole cell area was used to indicate the GFP-LC3 aggregation ratio (mean+/−SEM; n = 100 cells from 3 independent experiments; *, P < 0 .05, NS: no significance). (C) HeLa cells were transfected with FUNDC1 and mutants for 24 h, and then subjected to western blotting. (D) HeLa cells were transfected with scrambled or OPA1 knockdown shRNA vectors for 24 h and were then transfected with FUNDC1-MYC or FUNDC1 mutants for 24 h. The cells were fixed and immunostained to detect MYC (red) and HSP60 (purple). Scale bar: 10 µm. (E) The GFP-LC3 aggregates in cells treated as in (D) were quantified with imageJ. The GFP-LC3 aggregation area vs. whole cell area was used to indicate the GFP-LC3 aggregation ratio (mean+/−SEM; n = 100 cells from 3 independent experiments; *, P < 0 .05). (F) Scrambled shRNA-treated or DNM1L-KD cells were transfected by FUNDC1 for 24 h and then subjected to western blotting.