Table 1.
Antibiotic sensitivity testing results
| ID | Identificationa | Disk diffusionb | E-test | ESBLc | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATM | CAZ | CIP | IMP | TGC | C | D | G | GM | K | SAM | IMP | Phenotype | CTX-M | ||
| Clinical isolates | |||||||||||||||
| SL122 | Citrobacter freundii | R | R | R | S | S | R | R | R | R | R | R | 0.250 | YES | YES |
| SL124 | R | I | R | S | S | R | R | R | R | R | R | 0.380 | YES | YES | |
| SL127 | R | I | R | S | I | R | R | R | R | R | R | 0.380 | YES | YES | |
| SL129 | S | I | R | S | I | R | R | R | R | I | R | 0.190 | N/D | NO | |
| SL150 | R | R | R | S | I | I | R | R | R | R | R | 0.190 | YES | YES | |
| SL151 | R | I | R | S | S | R | R | R | R | I | R | 0.380 | YES | YES | |
| SL153 | R | R | R | I | I | R | R | R | R | I | R | 0.250 | YES | YES | |
| SL154 | R | R | R | I | S | R | R | R | R | I | R | 0.190 | N/D | NO | |
| SL155 | R | R | R | S | S | R | R | R | R | I | R | 0.250 | N/D | NO | |
| SL156 | R | R | R | I | I | R | I | R | R | R | R | 0.250 | YES | YES | |
| SL157 | R | R | R | S | I | R | I | R | R | R | R | 0.250 | YES | YES | |
| SL170 | R | I | R | S | S | R | R | R | R | I | R | 0.750 | YES | YES | |
| SL172 | R | R | R | S | S | S | R | R | R | R | I | 0.190 | YES | YES | |
| SL173 | R | R | R | I | S | R | R | R | R | I | R | 0.380 | YES | YES | |
| SL177 | R | R | R | S | S | R | R | R | R | I | R | 0.250 | YES | YES | |
| SL187 | R | I | R | S | S | S | I | R | R | I | I | 0.250 | YES | YES | |
| SL193 | R | I | R | S | S | R | I | R | R | S | R | 0.250 | YES | YES | |
| SL194 | R | R | R | S | S | R | R | R | R | I | R | 0.380 | N/D | NO | |
| SL197 | R | R | R | S | S | S | I | R | R | I | I | 0.250 | YES | YES | |
| SL200 | R | R | R | S | I | S | R | R | R | R | R | 0.190 | YES | YES | |
| SL202 | R | R | R | S | I | S | R | R | R | R | R | 0.190 | YES | YES | |
| SL205 | R | R | R | I | I | S | R | R | R | R | I | 0.190 | YES | YES | |
| SL160 | Enterobacter cloacae | S | S | S | S | S | S | S | R | S | S | S | 0.380 | NO | NO |
| SL161 | R | R | I | I | I | R | S | R | R | R | R | 0.250 | YES | YES | |
| SL167 | I | I | I | S | S | R | I | R | R | I | R | 0.380 | YES | YES | |
| SL168 | R | R | I | I | S | R | I | R | R | I | R | 2.000 | YES | YES | |
| SL174 | R | I | I | S | S | R | I | R | R | R | I | 0.380 | YES | YES | |
| SL175 | I | I | S | S | S | R | S | R | R | R | R | 0.250 | YES | YES | |
| SL179 | R | R | R | S | S | R | I | R | R | R | R | 0.250 | YES | YES | |
| SL181 | S | S | S | S | S | R | S | R | R | I | I | 0.380 | YES | YES | |
| SL182 | R | R | I | I | S | R | S | R | R | I | R | 0.380 | YES | YES | |
| SL189 | S | S | I | S | S | R | S | R | R | I | I | 0.250 | YES | YES | |
| SL192 | S | S | I | S | S | R | R | R | R | S | S | 0.380 | NO | NO | |
| SL195 | I | I | S | S | S | R | S | R | R | I | I | 0.380 | YES | YES | |
| SL198 | R | R | S | S | S | R | S | R | R | I | R | 0.250 | YES | YES | |
| SL199 | R | I | I | S | S | R | S | R | R | I | I | 0.380 | YES | YES | |
| SL203 | I | I | I | S | S | R | S | R | R | I | I | 0.380 | YES | YES | |
| SL115 | Escherichia coli | S | S | S | S | S | S | S | S | S | S | S | 0.190 | NO | NO |
| SL121 | S | S | R | S | S | R | R | R | S | S | I | 0.190 | NO | NO | |
| SL125 | S | S | S | S | S | S | S | S | S | S | S | 0.190 | NO | NO | |
| SL133 | S | S | S | S | S | S | R | R | S | S | R | 0.190 | NO | NO | |
| SL137 | S | S | S | S | S | S | R | R | S | S | R | 0.190 | NO | NO | |
| SL152 | S | S | I | S | S | R | R | R | R | S | S | 0.190 | NO | NO | |
| SL158 | S | S | R | I | S | R | R | R | S | S | R | 0.190 | NO | NO | |
| SL159 | S | S | S | I | S | S | S | S | S | S | S | 0.190 | NO | NO | |
| SL169 | S | S | S | S | S | R | R | R | S | S | S | 0.250 | NO | NO | |
| SL171 | S | S | S | S | S | R | I | R | S | S | R | 0.250 | NO | NO | |
| SL176 | S | S | R | S | S | S | S | R | S | S | I | 0.250 | NO | NO | |
| SL178 | R | S | S | S | S | R | I | R | I | S | S | 0.250 | NO | NO | |
| SL188 | S | S | S | S | S | R | I | R | S | S | S | 0.250 | NO | NO | |
| SL166 | Escherichia hermannii | R | R | I | S | S | R | S | R | R | I | R | 0.190 | YES | YES |
| SL108 | Klebsiella pneumoniae | S | S | I | S | S | S | S | S | S | S | S | 0.250 | NO | NO |
| SL113 | S | S | I | S | S | R | I | R | R | R | S | 0.250 | YES | YES | |
| SL116 | S | S | I | S | S | S | S | S | S | S | S | 0.190 | NO | NO | |
| SL136 | S | S | S | S | S | R | S | R | S | S | S | 0.250 | NO | NO | |
| SL141 | S | S | S | S | S | S | S | S | S | S | S | 0.250 | NO | NO | |
| SL162 | R | R | R | I | I | R | R | R | R | I | R | 0.190 | YES | YES | |
| SL163 | R | R | I | I | I | R | R | R | R | S | R | 0.190 | YES | YES | |
| SL164 | S | S | I | S | S | R | R | R | R | S | R | 0.190 | YES | YES | |
| SL165 | I | I | I | S | S | R | R | R | R | I | R | 0.250 | YES | YES | |
| SL180 | S | S | S | S | S | R | S | R | R | I | I | 0.380 | YES | YES | |
| SL183 | S | S | S | S | S | R | R | R | S | S | I | 0.250 | NO | NO | |
| SL185 | I | I | R | S | S | S | I | R | R | R | I | 0.190 | YES | YES | |
| SL186 | I | I | I | S | S | R | R | R | R | S | R | 0.250 | YES | YES | |
| SL190 | S | S | R | I | I | R | R | R | S | R | R | 0.250 | NO | NO | |
| SL196 | R | R | R | I | I | S | I | R | R | R | I | 0.250 | YES | YES | |
| SL184 | Enterobacter sp./Leclercia sp. | I | I | R | S | S | R | S | R | R | I | I | 0.250 | YES | YES |
| SL191 | I | R | R | I | I | R | S | R | R | I | I | 0.250 | YES | YES | |
| SL201 | R | R | R | R | I | R | S | R | R | R | I | 0.250 | YES | YES | |
| SL204 | R | R | I | R | I | R | R | R | R | R | I | 0.190 | YES | YES | |
| Environmental isolates | |||||||||||||||
| SL512 | Enterobacter cloacae | S | S | S | I | S | S | R | R | S | S | S | 0.250 | NO | NO |
| SL532 | I | I | S | S | S | R | S | R | R | R | R | N/A | YES | YES | |
| SL513 | Klebsiella pneumoniae | R | R | S | S | S | S | S | R | R | I | R | 0.190 | YES | YES |
| SL531 | S | S | S | S | S | S | S | S | S | S | S | 0.190 | NO | NO | |
| SL522 | Pantoea dispersa | S | S | S | S | S | S | S | S | S | S | S | 0.250 | NO | NO |
aConsensus identification based on combination phenotypic (API20E/ID32E) and 16S rRNA and rpoB gene sequencing
bDisk diffusion testing using the following antibiotics: ATM aztreonam, CAZ ceftazidime, CIP ciprofloxacin, IMP imipenem, TGC tigecycline, C chloramphenicol, D doxycycline, G sulfisoxazole, GM gentamicin, K kanamycin, SAM ampicillin/sulbactam. R resistant, I intermediate, and S sensitive phenotypes were determined based on CLSI criteria
cESBL phenotypes: YES – ESBL detected, NO – no ESBL present, N/D – ESBL presence could not be determined based on the E-test or double disk diffusion assay