Fig. 10.

d-γ-Tocopherol supplementation of allergic female mice increased the numbers of CD11c⁺CD11b⁺ DCs in the fetal liver. The mice were treated as in Fig. 2 and fetal livers were collected on GD18. The liver cells were isolated, immunolabeled and examined by flow cytometry. A: chart of lung CD11c⁺ subsets analyzed in the GD18 fetal liver. B: numbers of CD45⁺CD11c⁺CD11b-Ly6c⁺CD103-PDCA⁺MHCII⁺ plasmacytoid DC phenotype in the fetal liver. *P < 0.05, compared with corresponding mothers with basal diet. Ca-Cc: numbers of mDC subsets per million fetal liver cells. Cd-Cf: MFI of MHCII, CD80 and IRF4 on fetal liver cells with the mDC phenotype. Dg-Di: numbers of resident DC subsets per million fetal liver cells. Dj-Dl: MFI of MHCII, CD80, and IRF4 on fetal liver cells with the resident DC phenotype. Presented is the mean ± SE. *P < 0.05, compared with basal groups. **P < 0.05 compared with all other groups; n = 8–10 mice/group.