Fig. 11.

d-γ-Tocopherol increased the generation of bone marrow-derived IRF4⁺CD11c⁺CD11b⁺ DCs in vitro. Bone marrow from PND10 pups with basal diet was cultured for 10 days with GM-CSF in the presence of 0.01% DMSO (solvent control) or 2 μM d-γ-tocopherol (as we previously described for in vitro cell loading with d-α-tocopherol) (7). The cells were nonstimulated or stimulated with 20 μg/ml house dust mite (HDM) extract overnight. The DCs were analyzed by immunolabeling and flow cytometry. Presented is the number of IRF4⁺CD45⁺CD11b⁺CD11c⁺ MHCII⁺ DCs. There was no difference in the %live cells between the 2 groups (data not shown). There were no cells from the culture with the monocyte-derived phenotype (CD45⁺CD11c⁺CD11b⁺Ly6c⁺, MHCII high; data not shown); n = 5–6 from a representative experiment of 2 experiments. Presented is the mean ± SE. *P < 0.05, compared with all groups. **P < 0.05, compared with nonstimulated-DMSO group. #P < 0.05, compared with nonstimulated-DMSO control group and compared with HDM-stimulated γ-tocopherol-treated group.