Fig. 2.

Schematic of the timeline for d-α-tocopherol diet and ovalbumin (OVA) treatment of mothers and pups. Mothers were sensitized and challenged with OVA or saline, and then at the time of breeding, they were given basal diet or diet supplemented with 250 mg d-γ-tocopherol (d-γ-T)/kg of diet. On day 3 after birth, the pups were sensitized once with OVA/alum by intraperitoneal injection and then challenged with aerosolized 3% OVA on days 10–12. On postnatal day (PND) 13, the pup livers were analyzed for tocopherol isoforms by HPLC and the pup lungs were analyzed for bronchoalveolar lavage (BAL) inflammation and lung tissue dendritic cell (DC) subsets by multicolor flow cytometry. With another set of mothers, on gestational day (GD) 18, tocopherols were determined in the liver of the mothers by HPLC and the fetal liver was analyzed for DC subsets.