Fig. 11.

Effect BDL on recruitment of NF-κB p65 and cAMP response element binding protein (CREB)-binding protein (CBP)/p300 to NF-κB binding site in Ostα and Ostβ promoters. In vivo ChIP assays of mouse liver after 3 days of common BDL or after sham surgery in male C57BL6 mice using antibodies to NF-κB p65, CBP/p300, and RNA polymerase II. On quantitative RT-PCR there was a markedly increased recruitment of NF-κB p65 and CBP/p300 to the NF-κB binding site in Ostα (A) and Ostβ (B) promoters in BDL mice compared with controls. In contrast there was significant depletion of CBP/p300 at the FXRE of Ostα (C) and Ostβ (D) in these mice compared with control. *P < 0.05.