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Effect of siRNA knockdown of NF-κB p65 on endogenous expression of BSEP and FXR mRNA in Huh-7 cells. Immunoblot analysis (A) of Huh-7 cell extracts confirms siRNA depletion of NF-κB p65 protein compared with cells treated with a control siRNA. Knockdown of NF-κB p65 (B) is associated with a significant increase in both FXR and BSEP mRNA levels (C and D) in Huh-7 cells compared with cells transfected with a nonsilencing siRNA as assessed by real-time quantitative PCR. *P < 0.05.
