Fig. 7.

The corepressor silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) acts synergistically with NF-κB p65 to suppress ligand-induced transactivation of the BSEP promoter. Huh-7 cells were transiently transfected with FXR/RXR and plasmid vectors containing the BSEP- promoter sequence that included the NF-κBE linked to luciferase as reporter. Transfection with FXR/RXR in the presence of the FXR ligand GW4064 led to a marked transactivation of these promoters. NF-κB p65 markedly suppressed ligand-induced transactivation of the FXR promoter. SMRT but not nuclear receptor corepressor (NCoR) expression further enhanced the inhibitory effect of NF-κB p65. #P < 0.05.