Fig. 8.

Chromatin recruitment of NF-κB p65, SMRT, and histone deacetylases (HDACs) to the Bsep promoter after common bile duct ligation or treatment with LPS in mice. In vivo ChIP analysis of the Bsep promoter using mouse liver after 3 days of common bile duct ligation (BDL) or after sham surgery (A) and 6 h after treatment with lipopolysaccharide (LPS; 2.0 mg/kg; B) or saline vehicle by intraperitoneal injection in male C57BL6 mice using antibodies to NF-κB p65, SMRT, NCoR, HDAC1, HDAC2, HDAC3, and RNA polymerase II. On in vivo ChIP assays using quantitative RT-PCR there was a markedly increased recruitment of NF-κB p65 to the Bsep promoter in both BDL and LPS treated mice compared with controls. There was also increased recruitment of the corepressor SMRT but not NCoR to the NF-κB site. Additionally increased recruitment of HDAC 2 and 3 but not HDAC1 to this locus was observed. *P < 0.05.