Table 1.
Oligonucleotides for EMSA, ChIP assays, and primer sets for RT-PCR
| Gene | Sequence 5′-3′ |
|---|---|
| Primers used for qPCR analysis | |
| hBSEP | |
| Forward | aca tgc ttg cga gga cct tta |
| Reverse | gga ggt tcg tgc acc agg ta |
| hFXR | |
| Forward | gac ttt gga cca tga aga ccag |
| Reverse | gcc cag acg gaa gtt tct tatt |
| hOSTα | |
| Forward | ctg ggc tcc att gcc atc tt |
| Reverse | cac ggc ata aaa cga ggt gat |
| hOSTβ | |
| Forward | gca gct gtg gtg gtc att at |
| Reverse | tag gct gtt gtg atc ctt gg |
| hNF-κB p65 | |
| Forward | ccc cac gag ctt gta gga aag |
| Reverse | cca ggt tct gga aac tgt gga t |
| h36B4 | |
| Forward | gca atg ttg cca gtg tct gt |
| Reverse | gcc ttg acc ttt tca gca ag |
| Primers used in ChIP analysis | |
| hBSEP/FXRE site | |
| Forward | ggg ttt ccc aag cac act ctg tgt tt |
| Reverse | gag gaa gcc aga gga aat ggt gg |
| hBSEP/NF-κBE site | |
| Forward | atg ttc ttt tag ggt att tgt ctc |
| Reverse | tac agg cct gta gtt gtg aaa agt tac |
| mBsep/NF-κBE site | |
| Forward | gaa gag tcg ggc ctc tca cca ggc t |
| Reverse | agt cca gat cta gca cag ttc agt g |
| mOstα/NF-κBE site | |
| Forward | agc tct gac act tag atg cta cac |
| Reverse | gcc acc atg cct ggc ttc ta |
| mOstβ/NF-κBE site | |
| Forward | tgg tct ggc ctg cct cga tag |
| Reverse | tgg cga agg tca tga tat gaa c |
| Primers used in control ChIP analysis | |
| hBSEP | |
| Forward | aag cac tgg ccc atc aat tg |
| Reverse | ctc cta agg tgt aac aac t |
| mBsep | |
| Forward | ctc gag att tca cac aag tct aac aac t |
| Reverse | gtt cct gaa atg agg tta gtt |
| mOstα | |
| Forward | tag atg tgg agc ctt gat gag c |
| Reverse | atg gta cag atg gat gga g |
| mOstβ | |
| Forward | tgg gcc tgc ttc ctc ctc |
| Reverse | cag gaa gga gtc aag gct ct |
| Primers used in EMSA analysis | |
| hBSEP wt | |
| Forward | caa cca ggg att ttc caa gag ca |
| Reverse | tgc tct tgg aaa atc cct ggt tg |
| hBSEP mut | |
| Forward | caa cca gcc att ttc caa gag ca |
| Reverse | tgc tct tgg aaa atg gct ggt tg |
| Sequences of siRNAs used in this study | |
| hNF-κB p65 (catalog no. L-003533-00-0005; GE Dharmacon) | |
| siRNA pool 1 | GGAUUGAGGAGAAACGUAA |
| siRNA pool 2 | CCCACGAGCUUGUAGGAAA |
| siRNA pool 3 | GGCUAUAACUCGCCUAGUG |
| siRNA pool 4 | CCACACAACUGAGCCCAUG |
| Nontargeting siRNAs (catalog no. D-001810-01-20; GE Dharmacon) | |
| siRNA pool 1 | UGGUUUACAUGUCGACUAA |
| siRNA pool 2 | UGGUUUACAUGUUGUGUGA |
| siRNA pool 3 | UGGUUUACAUGUUUUCUGA |
| siRNA pool 4 | UGGUUUACAUGUUUUCCUA |
EMSA, electromobility shift assay; ChIP, chromatin immunoprecipitation; qPCR, quantitative polymerase chain reaction.