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. 2016 Feb 11;310(8):G599–G608. doi: 10.1152/ajpgi.00210.2015

Fig. 2.

Fig. 2.

A: LA CS induces Erk1/2 MAPK phosphorylation in Caco-2 cells. Caco-2 cells were incubated with LA CS (1:10 dilution) in serum-free cell culture medium for 1, 3, or 24 h. Extracted proteins (75 μg) were subjected to Western blot analysis on a 12% SDS-polyacryalmide gel utilizing phosphospecific Erk1/2 MAPK antibody (pErk1/2 MAPK). Blots were stripped and reprobed with the Erk1/2 MAPK antibody (total Erk1/2 MAPK) to indicate equal loading of protein in each lane. A representative blot of 3 different experiments is shown. Data were quantified by densitometric analysis. Values are means ± SE of 3 separate experiments. *P < 0.05, **P < 0.001 vs. respective untreated control (0 min). B: LA CS induces c-Fos or c-Jun protein expression in Caco-2 cells. Caco-2 cells were incubated with LA CS (1:10 dilution) in serum-free cell culture medium for 24 h. Extracted proteins (75 μg) were subjected to Western blot analysis on 10% or 12% SDS-polyacryalmide gel utilizing specific c-Fos or c-Jun antibody. Blots were stripped and reprobed with GAPDH antibody to indicate equal loading of protein in each lane. A representative blot of 3 different experiments is shown. Data were quantified by densitometric analysis. Values are means ± SE of 3 separate experiments. *P < 0.05 vs. untreated control (0 min).