Table 2.
Maximum isoprene production per day | Expression cassette localized on … | Reference |
---|---|---|
50 µg/g DCW | Chromosome | [4] |
67 µg/g DCW | Chromosome | [5] |
250 µg/g DCW | Chromosome | [6] |
63 µg/g DCW (pH 10) | Chromosome | [7] |
25 µg/g DCW (pH 10 + 600 mM NaCl) | Chromosome | [7] |
93 µg/g DCW (0 % NaCl) | Plasmid | This study (# 642 in the closed system) |
71 µg/g DCW (4 % NaCl) | Plasmid | |
336 µg/g DCW (0 % NaCl) | Plasmid | This study (# 642 in the open system) |
The isoprene values of Chaves et al. [7] and of Bentley and Melis [5] were recalculated to the dry weight basis using the reported relation of OD750 to dry cell weight (DCW). The calculation of the dry cell weight in our study based on the determined relation OD750 to dry cell weight (DCW) shown in the Additional file 10