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Overexpression of the tRNA encoding gene PFL_3991 increased pyoluteorin production by P. protegens Pf-5. PFL_3991 was expressed from a constitutive promoter in plasmid p6010-Arg. Pyoluteorin production of the wild-type strain containing p6010-Arg, denoted with an asterisk, was significantly higher (student t-test, p < 0.01) than the wild-type containing the empty vector pME6010. Values represent the average of three replicates and error bars show the standard deviation.
