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. 2016 Apr 19;7:511. doi: 10.3389/fmicb.2016.00511

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Copyright © 2016 Kubota, Kobayashi, Nunoura, Maruyama and Deguchi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of valine concentrations in the medium on the growth of Nautella sp. strain A04V. The substrates used for cultivation were: 6 mM D-Val (A), 6 mM racemic Val at D:L = 5:1 mM (B), D:L = 4:2 mM (C), D:L = 3:3 mM (D), D:L = 2:4 mM (E), D:L = 1:5 mM (F), and 6 mM L-Val (G) as the sole carbon and nitrogen sources. The concentrations of D-Val (filled circles) and L-Val (open circles) were determined by HPLC. The growth of the strain was measured instrumentally by determining the optical density at 660 nm (OD₆₆₀) (open triangles).