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. 2016 Apr 19;4:21. doi: 10.1186/s40425-016-0125-1

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© Siddiqui et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression of Fractalkine/CX3CL1 in human colon cancer tissues and cell lines. a Immunostaining score of Fractalkine/CX3CL1 in 171 patients with colorectal cancer; numbers on top of bars define exact number of patients with a given score; b Immunostaining of Fractalkine/CX3CL1 in human normal colonic mucosa (top) and in a representative human colon cancer tissue (bottom); c Immunofluorescence by confocal microscopy of Fractalkine/CX3CL1 protein expression in NCI-H630 cells and d in RKO cells upon transduction of the Fractalkine cDNA (CX3CL1-cherry) or mock-transduced (mock-cherry); e Quantification by ELISA of Fractalkine/CX3CL1 levels in the lysate of NCI-H630 tumors grown in NSG mice, normal skin tissue was used as reference control; f ELISA of Fractalkine/CX3CL1 levels in tumor lysates obtained from RKO tumors (RKO-MOCK and RKO-CX3CL1) grown in NSG mice