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. 2016 Apr 19;4:21. doi: 10.1186/s40425-016-0125-1

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© Siddiqui et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Transduction of the CX3CR1 receptor in human T cells and functional validation. a Population of CD3+ and of CD4+/CD8+ T cells post retroviral transduction of eGFP/CX3CR1 or -eGFP gene after 10 days of in vitro culture with hIL-2 medium. b Representative flow cytometry plot showing the transduction efficiency in eGFP or CX3CR1-eGFP in human T cells. Cells were gated on live cells followed by subsequent gating on CD3, CD4 and CD8 positive T cells. c mRNA expression of CX3CR1 in transduced CD3/IL-2 activated human T cells (Bars, Triplicates +/−SEM *P < 0.05, **P < 0.01 for difference between eGFP and CX3CR1-eGFP-T cells (Student’s t test). d Transwell migration assay of eGFP-T cells or CX3CR1-eGFP T cells in response to different concentrations of rhCX3CL1, ****P < 0.0001, Two-way ANOVA