FIG 2.

Domain alignment of yeast and human centrin-binding proteins. The centrin-binding motifs (marked with W, Y, L, M, V, or F) of ScSfi1, SpSfi1, hPOC5 (isoform 1), and hSFI1 (isoform a) are shown schematically. The predicted centrin binding consensus motif of SFI1 molecules is shown in the box above ScSfi1. In addition, centrin (pink) binding to hSFI1 is indicated. Structural data suggest that each centrin binds to binding sites at SFI1 at a 60° angle with respect to the precursor centrin. Cyclin-dependent kinase and polo-like kinase phosphorylation sites within the C terminus of ScSfi1, SpSfi1, hPOC5, and hSFI1 were either published (ScSfi1) or predicted by the GPS software. Purple asterisk, confirmed Cdk1 site; blue asterisk, predicted cyclin-dependent kinase site; pink asterisk, predicted polo-like kinase site.